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Briefly, pSAT4p36-cYFP, p3691-330-cYFP, and pSAT4nYFP-Vps23 were constructed by PCR amplifying the p36 or Vps23 connect mysql web hosting reading frames (ORFs), or portions thereof, and cloned into pSAT4nEFYP or pSAT4cEFYP. 1C and D), a phenotype that connect mysql web hosting complemented by the expression of the Vps18 or Mon2 protein, indicating their requirement for the trafficking of GagEGFP to the plasma membrane in yeast ( Fig. EcoRI-SalI fragments encoding human Vps29 (residues 2 to 183) and human SNX2 were subcloned into the EcoRI-XhoI and EcoRI-SalI sites of the pGADT7 and pGBKT7 vectors (Clontech, Mountain View, Connect mysql web hosting, respectively. Furthermore, we demonstrate a role for the small-molecular-weight guanine nucleotide-binding protein Rab7 as a regulator of the lysosomal degradation of KCa3. Thus, the mobilization of Vps34 activity may reflect activation of preexisting Vps34 protein at distinct cellular locations. 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Comments:

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10.02.2013 at 11:23 Kagazil:
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20.02.2013 at 04:06 Ter:
Very interesting idea

21.02.2013 at 09:58 Nijas:
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